Dr Kim Kaufman
Director of Brain Cancer Research
BMedSc (Hon 1), PhD (Medicine)
Phone: (02) 8514 0675
Dr Kim Kaufman is a neuroscientist and cancer researcher passionate about improving the quality of life and survival rates for brain cancer patients.
Kim graduated with a Bachelor of Medical Science (Hons Class I, Neuropathology) at the University of Sydney in 2003. As a biomedical scientist, she has a broad skillset that spans multiple research areas including oncology, neuroscience, pathology and biochemistry. Kim contributed to the field of clinical proteomics since 2004, publishing some of the first human brain tissue studies in 2006. She completed an internship at the RIKEN Brain Science Institute, Japan in 2006 and was awarded a PhD in Medicine (Neuropathology) in 2008, for which she received Sydney University’s ‘Bercovici Prize for Excellence in Medical Research’ as well as the ‘Bannigan Prize for the Best PhD in Neuroscience’ from the Nerve Research Foundation.
Her promise as a future leader in cancer research has been recognised by two career support grants, including an NHMRC Australian Biomedical Fellowship (2011-2015) and Cancer Institute NSW Early Career Research Fellowship (2014-2018). Her research has been presented at multiple National and International conferences, receiving several grants and presentation awards.
She holds an Honorary Senior Research Fellow title with the University of Sydney and am an Honorary Senior Research Officer in the Neuropathology Department, Royal Prince Alfred Hospital. Dr Kaufman works across campuses in this important clinical and translational research hub; my brain cancer laboratory is co-located with RPAH Neuropathology at the Brain and Mind Centre, Sydney University.
Supervisor of postgraduate and undergraduate research students in PhD, Masters and Honours degree programs.
Kim has supervised 5 PhD students (including 4 completions) and was primary supervisor of the University Medal BMedSc(Hon I) recipient in 2016.
Dr Kaufman’s research uses cutting-edge mass spectrometry and gene sequencing studies to resolve the molecular landscape of brain cancer. This highly complex molecular information is used to understand how tumours invade and hijack their microenvironments, identify therapeutic targets and chemo-resistance mechanisms, and resolve novel, clinically-relevant biomarkers.
She is the Manager of the multi-site Sydney Brain Tumour Bank and have expanded our biobanking collections to capture blood, living tumour cells and postmortem brains to support a range of translational brain cancer research projects.
NeuroOncology NSW Oncology Group, Cooperative Group for NeuroOncology (COGNO), MAGMA Trial Translational Sub-comittee, Brain Cancer Biobanking Australia, The Australian and New Zealand Society for Neuropathology, NHMRC Research Translation Faculty, European Association for Cancer Research, Australian Proteomics Society, Cancer Research Network Sydney University, Cancer Research Network – Brain Tumour and Tumour Microenvironment SIGs, Sydney Catalyst, Experimental Neuropathology Affinity Group, Sydney Neuroscience Network.
KK34 S. Hallal, S. Ebrahimkhani, H. Wei, M. Lee, H. Sim, J. Sy, B. Shivalingam, M.E. Buckland and K.L. Alexander-Kaufman “Deep sequencing of small RNAs from neurosurgical extracellular vesicles substantiates miR-486-3p as a circulating biomarker that distinguishes glioblastoma from lower-grade astrocytoma patients” International Journal of Molecular Science 2020.
KK33 S. Hallal, A. Azimi, H. Wei, N. Ho, M. Lee, H. Sim, J. Sy, B. Shivalingam, M.E. Buckland and K.L. Alexander-Kaufman “A comprehensive proteomics SWATH-MS workflow for profiling blood extracellular vesicles reveals an exciting new avenue for glioma tumour surveillance” International Journal of Molecular Science 2020
KK32 A. Azimi, K.L. Kaufman, J. Kim, M. Ali, G.J. Mann and P. Fenandez-Penas, “Proteomics: an emerging approach for the diagnosis and classification of squamous cell carcinomas and its precursors” Journal of Dermatological Science 2020. Cit=1
KK31 A. Azimi, P. Yang, V. Howard, G.J. Mann, K.L. Kaufman and P. Fernandez-Penas, “Data independent acquisition proteomics analysis can discriminate between actinic keratosis, Bowen’s disease and cutaneous squamous cell carcinoma” Journal of Investigative Dermatology doi: 10.1016/j.jid.2019.06.128. 2020.
KK30 S. Hallal, S. Ebrahimkhani, B. Shivalingam, M. Graeber, M.E. Buckland and K.L. Kaufman, “The emerging clinical potential of circulating extracellular vesicles for non-invasive glioma diagnosis and disease monitoring” Brain Tumor Pathology 2019
KK29 S. Hallal, D.M. Mallawaaratchy, H. Wei, S. Ebrahimkhani, B.W. Stringer, B.W. Day, A.W. Boyd, G.J. Guillemin, M.E. Buckland and K.L. Kaufman, “Extracellular vesicles released by glioblastoma cells stimulate normal astrocytes to acquire a tumor-supportive phenotype via p53 and MYC signaling pathway” Molecular Neurobiology, 2019
KK28 S. Ebrahimkhani, F. Vafaee, S. Hallal, H. Wei, M.Y.T. Lee, P.E. Young, L. Satgunaseelan, H. Beadnall, M.H. Barnett, B. Shivalingam, C.M. Suter, M.E. Buckland and K.L. Kaufman, “Deep sequencing of circulating exosomal microRNA allows non-invasive glioblastoma diagnosis” npj Precision Oncology 2018. KK27 S. Hallal, B.P. Russell, H. Wei, M. Lee, C.W. Toon, J. Sy, B. Shivalingam, M.E. Buckland and K.L. Kaufman, “Proteome profiling of extracellular vesicles from neurosurgical aspirates identifies Chaperonin Containing TCP1 Subunit 6A (CCT6A) as a potential glioblastoma biomarker with prognostic significance” Proteomics, Special Issue: Extracellular Vesicles and Exosomes 2018.
KK26 A. Azimi, M. Ali, K.L. Kaufman, G.J. Mann, P. Fernandez-Penas, “Tape stripped stratum corneum samples prove to be suitable for comprehensive proteomic investigation of actinic keratosis” Proteomics: Clinical applications, 2018.
KK25 A. Azimi, K.L. Kaufman, M. Ali, S. Kossard, P. Fernandez-Penas, “Differential proteomic analysis of actinic keratosis, Bowen’s disease and cutaneous squamous cell carcinoma by label free LC-MS/MS” Journal of Dermatological Science 91(1):69-78 2018.
KK24 D.M. Mallawaaratchy, S. Hallal, B. Russell, L. Ly, S. Ebrahimkhani, H. Wei, R.I. Christopherson, M.E. Buckland, K.L. Kaufman, “Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease” Journal of NeuroOncology 131(2):233-44 2017
KK23 A. Azimi, K.L. Kaufman, M. Ali, S. Kossard, P. Fernandez-Penas, “In silico analysis validates proteomic findings of formalin-fixed paraffin embedded cutaneous squamous cell carcinoma tissue” Cancer Genomics Proteomics 13(6):453-65 2016,
KK22 P.Y. Huang, S. Mactier, N. Armacki, K.L. Kaufman, L. Belov, O.G. Best, D. Pascovici, S.P. Mulligan, and R.I. Christopherson, “Protein Profiles Distinguish Stable and Progressive Chronic Lymphocytic Leukemia” Leukemia and Lymphoma 16:1-11 2016.
KK21 K.L. Kaufman, Y. Jenkins, M. Alomari, O.G. Best, D. Pascovici, S. Mactier, S. Mulligan, P. Haynes and R.I. Christopherson, “The Hsp90 inhibitor SNX-7081 is synergistic with fludarabine nucleoside via DNA damage and repair mechanisms in human, p53-negative chronic lymphocytic leukemia” Oncotarget 6(38):40981-97 2015.
KK20 N.J Hare, B. Chan, E. Chan, K.L. Kaufman, W.J. Britton and B.M. Saunders, “Microparticles released from Mycobacterium tuberculosis-infected human macrophages contain increased levels of the type I interferon inducible proteins including ISG15” Proteomics 15(17):3020-9 2015
KK19 D.M. Mallawaaratchy, M.E. Buckland, K.L. McDonald, C.C.Y. Li, L. Ly, E.K. Sykes, R.I. Christopherson and K.L. Kaufman, “Membrane proteome analysis of glioblastoma cell invasion” Journal of Neuropathology and Experimental Neurology 75(5):425-41 2015.
KK18 J. Zhou, L. Belov, P. Chapuis, C. Chan, N. Armstrong, K.L. Kaufman, M.J. Solomon, S.J. Clarke and R.I. Christopherson, “Surface profiles of live colorectal cancer cells and tumour infiltrating lymphocytes from surgical samples correspond to prognostic categories” Journal of Immunological Methods 416:59-68 2015.
KK17 P. Wilcox, C.C. Li, M. Lee, B. Shivalingam, J. Brennan, C.M. Suter, K.L. Kaufman, T. Lum and M.E. Buckland “Oligoastrocytomas: throwing the baby out with the bathwater?” Acta Neuropathologica 129(1):147-9 2015.
KK16 K.L. Kaufman, S. Mactier and R.I. Christopherson, “Surface antigen profiling of surgical melanoma specimens” in Melanoma: Methods and Protocols, Methods in Molecular Biology 2015.
KK15 S. Mactier, K.L. Kaufman, P. Wang, B. Crossett, E.H. Yang, G.M. Pupo, R.A. Scolyer, J.H. Yang, G.J. Mann and R.I. Christopherson “Protein signatures correspond to survival outcomes of AJCC stage III melanoma patients” Pigment Cell and Melanoma Research 27(6):1106-16 2014. IF 2016=5.17
KK14 M. Alomari, S. Mactier, K.L. Kaufman, O. G. Best, S. P. Mulligan and R. I. Christopherson “Profiling the lipid raft proteome from human MEC1 chronic lymphocytic leukemia cells” Journal of Proteomics and Bioinformatics, Vol 7 “Affinity Proteomics”, 2014,
KK13 K.L. Kaufman, S. Mactier, N.J. Armstrong, D. M. Mallawaaratchy, S.N. Byrne, L.E. Haydu, V. Jakrot, J.F. Thompson, G.J. Mann, R.A. Scolyer and R.I. Christopherson “Surface antigen profiles of leukocytes and melanoma cells in lymph node metastases are associated with survival in AJCC stage III melanoma patients” Clinical and Experimental Metastasis 31(4):407-21 2014
KK12 Y. Che, O.G. Best, L. Zhong, K.L. Kaufman, S. Mactier, M. Raftery, L.M. Graves, S.P. Mulligan and R.I. Christopherson “Hsp90 inhibitor SNX-7081, dysregulates proteins involved with DNA repair and replication and the cell cycle in human chronic lymphocytic leukemia (CLL) cells” Journal of Proteome Research 12(4): 1710-22, 2013.
KK11 D. M. Mallawaaratchy, S. Mactier, K.L. Kaufman, K. Blomfield, and R.I. Christopherson, “The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells” Journal of Proteomics 75(5):1590-0, 2012.
KK10 E. Heath, K.L. Kaufman and R.I. Christopherson, “B-RAF: a contributor to the melanoma phenotype” Molecules in Focus, International Journal of Biochemistry and Cell Biology 43(1):29-32 2011.
KK9 N. Zahr, K.L. Kaufman and C. Harper, “Clinical and pathological features of alcohol-related brain damage” Nature Reviews Neurology 7, 284-94 2011
KK8 K.L. Kaufman, L. Belov, P. Huang, S. Mactier, R. Scolyer, G. Mann and R.I. Christopherson, “An extended antibody microarray for surface profiling metastatic melanoma” Journal of Immunological Methods 358(1-2): 23-34 2010.
KK7 K.L. Alexander-Kaufman and C. Harper, “Transketolase: observations in alcohol-related brain damage research” Molecules in Focus, International Journal of Biochemistry and Cell Biology 41:717-20 2009
KK6 H. Matsuda, T. Iwasaki, K.L. Alexander-Kaufman and I. Matsumoto “High-throughput analysis of alcoholic and drug abused postmortem human brain” Japanese Journal of Molecular Psychiatry (Bunshi Seishin) 6(3): 24-29 2009.
KK5 K.L. Alexander-Kaufman, S. Cordwell, C. Harper and I. Matsumoto “A proteome analysis of the dorsolateral prefrontal cortex in human alcoholic patients” Proteomics: Clinical Applications 1(1):62-72 2007
KK4 K.L. Alexander-Kaufman, I. Dedova, C. Harper and I. Matsumoto “Proteome analysis of the dorsolateral prefrontal region from healthy individuals” Neurochemistry International 51(6-7):433-9 2007
KK3 K.L. Alexander-Kaufman, C. Harper, P Wilce and I. Matsumoto “The cerebellar vermis proteome of chronic alcoholic individuals” Alcoholism: Clinical and Experimental Research, 31(8):1286-96 2007
KK2 I. Matsumoto, K.L. Alexander-Kaufman, T. Iwazaki, M. Kashem and H. Matsuda “Central nervous system proteomes in alcohol and drug abuse and dependence” Expert Review of Proteomics 4(4): 539-52 2007
KK1 K.L. Alexander-Kaufman, G. James, D. Sheedy, C. Harper and I. Matsumoto “Differential protein expression in the prefrontal white matter of human alcoholics: a proteomics study” Molecular Psychiatry 11(1): 56-65 2006
K.L. Kaufman, S. Mactier, P. Kohnke and R.I. Christopherson, “Cell surface oncoproteomics: cancer biomarker discovery and clinical applications”, in Oncoproteins: Types and Detection, Nova Science Publishers, Inc. Edited by Jeremy R. Davis (2011) ISBN: 978-1-61761-610-5
Matsumoto, K.L. Kaufman, M.A. Kashem and H. Matsuda-Matsumoto, “Ethanol-related Encephalopathies”, in Drug-related Encephalopathies, Nova Science Publishers, Inc. Edited by G. P. Sechi (2008) ISBN: 978-1-60456-745-8
Published Conference Abstracts:
- M. Batarseh, K. Ekroos, S. Hallal, K. Kaufman, “Enhancing extracellular vesicle isolation of human plasma verified by high resolution lipidomics” Journal of Extracellular Vesicles 8(Suppl 1): 1593587(2019)
- Hallal, D. Mallawaaratchy, H. Wei, M. Buckland, K. Kaufman, “Glioblastoma stem cells induce an invasive phenotype in normal astrocytes via extracellular vesicles”, Journal of Extracellular Vesicles 7, 236-236 (2018)
- Hallal, B. Russell, B. Shivalingam, M. Buckland, K. Kaufman, “Proteome analysis of glioma-derived extracellular vesicles isolated from neurosurgical aspirates provide markers for disease stage and progression”, Journal of Extracellular Vesicles 7, 57-58 (2018)
- Kim, B. Kong, T.H. Lo, F. Kupresanin, M. Papadimitrious, J. Hsu, P. Fromm, K. Kaufman, M. Buckland, G. Clark, D. Hart, “A combinatory immunotherapy against brain tumour: blood dendritic cell based vaccine therapy with checkpoint inhibitors”, European Journal of Immunology 48:161-161 (2018)
- Azimi, K.L. Kaufman, M. Ali, S. Kossard, P. Fernandez-Penas, “Label-free proteomic analysis of microdissected formalin-fixed and paraffin embedded cutaneous squamous cell carcinoma tissue”, Australiasian Journal of Dermatology, 57:49(1):S1 (2016)
- Mulligan, M. Alomari, K.L Kaufman, S. Mactier, O.G. Best, R. I. Christopherson, “Rituximab induces major changes in the surface proteome of lipid rafts on human MEC1 chronic lymphocytic leukemia cells”, Leukemia & Lymphoma 56(S1) (2015)
- M Mallawaaratchy, K.L. McDonald, E.K. Sykes, M.E. Buckland, R.I. Christopherson and K.L. Kaufman “Novel proteins associated with invadopodia structures in glioblastoma” NeuroOncology 16(Suppl 2) ii100 (2014)
- L. Kaufman, L. Ly, M. McKay, D.M. Mallawaaratchy, S. Mactier, B. Crossett, M. Molloy, M.E. Buckland, K.L. McDonald and R.I. Christopherson “Comprehensive proteomic profiling of bevacizumab-resistant glioblastoma” NeuroOncology 16(Suppl 2) ii98 (2014)
- M. Mallawaaratchy, R.I. Christopherson and K.L Kaufman “Membrane proteome analysis of invasive human glioblastoma multiforme” European Journal of Cancer 49(2): S132-133 (2013)
- Mactier, K.L. Kaufman, P. Wang, B. Crossett, E. Yang, R.A. Scolyer, J. Yang, G.J. Mann and R.I. Christopherson “Identification of markers prognostic of survival in lymph node metastases from stage III melanoma patients” European Journal of Cancer 49(2): S874 (2013)
- Harper, K.L. Alexander-Kaufman, M. Kashem, I. Matsumoto, X. He, A. Pfefferbaum and E. Sullivan “Thiamine deficiency is a critical factor in alcohol-related brain damage – new experimental structural and human proteomics data” Alcoholism: Clinical and Experimental Research 32(6):300A (2008)
- L. Alexander-Kaufman, M.A. Kashem, C. Harper, D. Sheedy and I. Matsumoto, “Proteome analyses of human brain regions sensitive to alcohol-induced brain damage” Alcoholism: Clinical and Experimental Research 31(6): 31A (2007)
- L. Alexander-Kaufman, C. Harper and I. Matsumoto, “Alcohol-related brain damage: proteomics investigations using postmortem human brain tissue” Neuropathology 27(2): 157-211 (2007)
- L. Alexander-Kaufman, C. Harper and I. Matsumoto, “Proteome analyses of human brain regions sensitive to alcohol-induced brain damage” Neuropathology 27(2): 157-211 (2007)
- L. Alexander-Kaufman, C. Harper and I. Matsumoto “A proteome analysis of the dorsolateral prefrontal cortex in human alcoholic patients” Alcoholism: Clinical and Experimental Research 30: 157A (2006)
- Matsumoto and Alexander-Kaufman “Alcohol-specific cascades underlying alcohol-induced prefrontal cortex damage: Insight into proteomics studies” Alcoholism: Clinical and Experimental Research 30: 76A (2006)
- Matsumoto and L. Alexander-Kaufman, “Alcohol-induced brain damage; proteomics on postmortem human brain” Alcoholism: Clinical and Experimental Research 30: 85A (2006)
- L. Alexander-Kaufman, G. James, D. Sheedy, C. Harper and I. Matsumoto, “Protein expression changes in prefrontal white matter from human alcoholics” Alcohol and Alcoholism Supplement 40(1): i1-63. (2005)
- L. Alexander, G. James, D. Sheedy, C. Harper and I. Matsumoto, “Differential protein expression in the white matter of the dorsolateral prefrontal region in human alcoholics” Journal of Neurochemistry 94 (Suppl. 2): 64-128 (2005)